Equipment and associated skills dedicated to the multi-scale characterization of the structure and composition of food matrices from bio-based or transformed sources:
✔️ Equipment accessible on the dairy technology research platform (PFL):
▶️ Own equipment installed on the dairy technology research platform (PFL):
These equipment are unitary and connected to each other to allow all the steps of milk transformation within a 400 m² technological hall completed by 2 cheese-making workshops (soft and cooked pressed pastes), 2 salting rooms, 1 brining room and 2 food preparation rooms.
The main equipment allows the realization of the steps of: (click on the active links for direct access)
- MILK PREPARATION: Equipment for skimming, homogenizing and heat treating milk.
- MEMBRANE SEPARATION: Equipment for microfiltration, ultrafiltration, nanofiltration and reverse osmosis.
- CHEESE TRANSFORMATION: Equipment for the manufacture of cheeses of the type soft pastes, uncooked pressed pastes, cooked pressed pastes as well as for the manufacture of processed cheeses and fresh cheeses.
- CONCENTRATION / DRYING: Equipment for the concentration and drying of dairy products.
For direct access to all the descriptions of PFL equipment: Click HERE
Presentation brochures of the available technologies (click on the active links): Filtration / Cheese technologies / Evaporation / Drying
▶️ Access to the equipment of the backing unit UMR Science and technology of milk and egg (STLO):
(i) Biological Resource Center dedicated to bacteria of food interest, which hosts more than 4000 strains of 99 different species for an optimal exploration of biodiversity.
(ii) Analytical tools in biochemistry, process and microbiology associated with the three main areas of expertise of the UMR STLO:
- Multi-scale exploration of food: mass spectrometry, Biacore, atomic force microscopy, isothermal titration calorimetry, differential scanning calorimetry, laser granulometry, infrared spectroscopy
- Exploration of the biodiversity and expression of microorganisms at the genomic and proteomic level and at the phenotypic level
✔️ Equipment available on the PLANET platform:
▶️ The Planet platform has equipment to deal with five themes:
- Dry refining of lignocellulosic biomass
↪ Examples of equipment: Knife mill, pallet mill, ball mill, pin mill, air jet mill; Turbo-separator; Electrostatic sorter…
- Fractionation, sorting and separation of seeds
↪ Examples of equipment: Optical sorter; Density table; Pilot semolina mill; Mills, Huller…
- Structuring of agrocomposites
↪ Examples of equipment: Co-rotating twin-screw extruders, Heated press; Injection press…
- Treatment and structuring of food products
↪ Examples of equipment: Mixers; Single-screw extruder; Co-rotating twin-screw extruder; Inclined rotary drum; Dryers…
- Characterization of materials
↪ Examples of equipment: Laser granulometer; Granulomorphometer; Colorimeters; Texturometer…
For direct access to all the descriptions of PLANET equipment: Click HERE
✔️ Equipment grouped within the PROBE Research Infrastructure:
▶️ BIBS: Platform for the structural characterization of biopolymers (polysaccharides, lipids, proteins), in biological systems (plants, plant organs, cells…) or synthetic and model systems (food and food matrices)
- Mass spectrometry laboratory:
- SL Ion Trap (Bruker, 2018): ESI-IT technology coupled with a UPLC
- Select SERIES Cyclic IMS (Waters, 2019): ESI-Q-IM-TOF technology coupled with a UPLC
- Q-Exactive HF (Thermo-Fisher, 2019): ESI-Q-Orbitrap technology coupled with a nano-UPLC
- RapifleX Tissue typer (Bruker, 2018): MALDI TOF technology
- Microscopy laboratory:
- Transmission electron microscope (JEOL 1230, 2005)
- Atomic force microscope (Bruker coupled with Raman Renishaw, 2013)
- Scanning electron microscope Quattro S (Thermo-Fisher, 2019)
- Laser scanning confocal microscope (NIKON A1R, 2009, partial rejuvenation in 2019)
- NMR laboratory:
- High field NMR spectrometer Avance III 9.4T WB (Bruker, 2002, electronic rejuvenation in 2009, new magnet in 2018)
- Two low field NMR spectrometers Minispec Mq20 0.47T (2009 and 2018, Bruker)
- Compositional and structural phenotyping laboratory:
- ASE 350 extractors (Thermo-Fisher, two extractors 2010 and 2012) and automated preparation platform (Swing SL robotic workflow station, Chemspeed, 2011)
- TRACE GC Ultra and TRACE ISQ (Thermo-Fisher, 2011)
- HPAEC, ICS 6000 (Thermo-Fisher) (2019)
- ACQUITY ELSD FLUO SQD2 (Waters) (2016 and 2020)
For direct access to all the descriptions of BIBS equipment: Click HERE
▶️ ChemoSens : physico-chemical and organoleptic characterization of food, analysis of lipids in food and in neurosensory tissues
- Tools for the physico-chemical characterization of food:
° Mass spectrometry pole:
- 2 gas chromatographs coupled to mass spectrometry, quadrupole, EI/CI sources, Agilent 7890A / MSD 5975C (GC-MS)
- 1 two-dimensional gas chromatograph, coupled to mass spectrometry, quadrupole, EI/CI source, and to olfactometry, Agilent, 2GC 7890A / MSD 5975C (GC-2D-MS/O)
- 1 mass spectrometer by proton transfer reaction, time of flight, Ionicon (PTR-ToF-MS)
- 1 tandem mass spectrometer, triple quadrupole, ESI/APCI source, Waters Xevo TQ-S micro (LC-QqQ-MS/MS) which can be coupled to two chromatographic systems:
A liquid chromatography (LC) chain: Acquity UPLC, Waters (LC-MS/MS)
A supercritical fluid chromatography (SFC) chain: Acquity UPC2, Waters (SFC-MS/MS)
- 1 high resolution mass spectrometer, Thermo Orbitrap Tribrid FUSION (HRMS) equipped with ion sources:
ESI/APCI with coupling to ultra high performance liquid chromatography (UHPLC-HRMS)
NanoSpray with coupling to high performance liquid nanochromatography (nanoLC-HRMS)
- APPI for its coupling to gas chromatography (GC-APPI-HRMS)
° Olfactometry pole:
- 2 gas chromatographs coupled to olfactometry, Agilent 7890B (GC-O)
- 2 Burghart OLO23MD olfactometers
- 1 threshold olfactometer
- Equipment for the sensory characterization of food:
- 4 sensory evaluation rooms, each with 16 computerized cabins
- 4 kitchens
- 1 preparation room
- 5 eye trackers
- TimeSens® software
- PanelSens a database of more than 10,000 people who have already done tastings in Dijon
- Other equipment:
- Liquid chromatograph coupled to a charged aerosol detector Thermo Corona (HPLC-CAD)
- Liquid chromatograph coupled to a diode array detector U3000 Diode Array (HPLC-DAD)
For direct access to all the descriptions of ChemoSens equipment: Click HERE
▶️ PFP: Platform for the analysis of polyphenols from fruits, plants, food, processed products
- Two high resolution mass spectrometers (HRMS):
- Orbitrap Exploris 480 (ThermoFisher), coupled with UHPLC- very large dynamic range, very high resolution (500k @200m/z), mass accuracy <1ppm in routine
- timsTOF (Bruker): mass and ion mobility spectrometer (IMS-Q-TOF), coupled with UHPLC
- A nuclear magnetic resonance (NMR) spectrometer:
- NMR 400MHz “liquid” (Bruker): automated for large-scale analysis and dedicated to wine authentication
For direct access to all the descriptions of PFP equipment: Click HERE
▶️ AgroResonance : NMR platform for agronomy, agri-food and nutrition
3 high-field magnetic spectrometers of the latest generation equipped with imaging gradients and high levels of spectral, spatial or temporal resolution:
- NMR spectrometer 11.7 T - Horizontal (imaging and localized spectroscopy in vivo in small animals)
- NMR spectrometer 9.4 T - Vertical (micro-imaging and spectroscopy in vitro)
- NMR spectrometer 4.7 T - Horizontal (imaging in large fields of view with or without physico-chemical constraints)
For direct access to all the descriptions of AgroResonance equipment: Click HERE
✔️ Equipment available on the technical platform for simulating the digestive environment (DIGEST-IV):
▶️ Models of gastrointestinal digestion (stomach & small intestine)
- 2 TIM1 systems, one of which is in a P3 level confined laboratory
- 1 ESIN system under development
▶️ Models of colonic fermentation simulation
- 15 ARCOL bioreactors, 5 of which are in P3 lab, for mono-compartmental use with or without mucosal phase, or tri-compartmental (ascending, transverse, descending colon)
▶️ Aerobic / anaerobic microbiology, cultural / molecular
- PSM, anaerobic hood, incubators, qPCR…
▶️ Analytical environment
- Micro-GC (TCD), HPLC (DAD, refractometer), electrophoresis…
For direct access to all the descriptions of DIGEST-IV equipment: Click HERE
